Trifonova A. Properties of cryopreserved cell cultures in regenerative media which contain low molecular fraction from cord blood.

The research object - recovery of morpho-functional properties of cell cultures after cryopreservation in the process of their cultivation with the fraction below 5 kDa extracted from the cattle cord blood. The research aim - study of the cattle cord blood fraction (below 5 kDa) and the comparator agent Actovegin effects on nonlethal injuries recovery in cells after cryopreservation. Research methods: cultivation сell cultures, cryopreservation cell suspensions, cytological, microscopically and statistical methods. The investigation of proliferation of native cell cultures and cryopreserved ones revealed a conspicuous stimulating effect of the cord blood fraction (below 5 kDa), which exceeded Actovegin effect and depended firstly on the type of cell culture investigated and secondly on the fraction quantity added. The ability of the cord blood fraction below 5 kDa and Actovegin to accelerate entry into division and to increase the mitotic activity of the cultures investigated was ascertained. The improvement of adhesion processes of cell cultures and the total morphology of cells when the cord blood fraction below 5 kDa or Actovegin were added to cultivation media was shown. Adding the cord blood fraction below 5 kDa or Actovegin significantly accelerated spreading both native cells and cryopreserved ones. Expediency of creation of regenerative media for cryopreserved cell cultures, in which the cattle cord blood fraction below 5 kDa is used as an active agent is proved.