Bilko D. Expression of the nuclear and Toll-like receptors and their functional activity in the tumor cells of different origin

Object: primary meningioma cultures and tumor cell lines. Aim: to determine the expression profiles of nuclear receptor superfamily and Toll-like receptors and their functional junctions in tumor cells of different origin and to investigate the regulation of cell death by cationic mitochondrial targeted compounds. Methods: cultural, polymerase chain reaction, Western-blotting, light, fluorescent, and transmission microscopy, biochemical, statistical. Results: It was determined that most commonly found coexpression association of the nuclear receptor superfamily members was glucocorticoid receptor with peroxisome proliferator activated receptor and TLR1-6. Action of the novel cationic compound, hexadecyl-triphenyle-phosphonium bromide, on the tumor cells was determined, that was able to induce apoptosis of the tumor cells at very low doses by directly causing damage to the mitochondria. Novelty: Criteria of NRS and TLR gene expression analysis in tumor cells were defined to assess tumor growth and its response to anticancer drugs. Molecular PCR-investigation of bioptats should be performed to determine the presence of glucocorticoid receptor in tumor. It is recommended to be taken into account to choose optimal scheme of medicament reduction of tumor cell mass. Fields of use: cell and molecular biology, biochemistry, oncology, pharmacology.