Kyrylenko O. Kyrylenko. Influence of modification of cellular membranes lipid composition on the flow development of ischemic heart disease: experimental and clinical research

The dissertation is devoted to studying the role of modification of fatty acid composition of cell membranes with omega-3 PUFA during ischemia-reperfusion and the study of mechanisms of cardioprotective effect of omega-3 PUFA in the experimental conditions and in patients with IHD. Experimental studies on isolated hearts of rats under outgoing conditions, ischemia-reperfusion, argued that the new substance of omega-3 polyunsaturated fatty acids of vegetable origin and omega-W PUFA of animal origin (Epadol) has cardioprotective ability. Clinical studies have shown that the addition to the basic treatment of omega-W PUFA (Epadol), stabilizes the IHD, improve the health status of patients with coronary artery disease, normalizes heart function, basic laboratory values, changes the composition of membranes of blood cells. It is established that modification of fatty acid composition of cell membranes of the myocardium significantly prevents heart failure in acute ischemia and subsequent reperfusion in experimental conditions. For the first time developed a method for predicting the dynamics of atherosclerosis and coronary heart disease, as well as the model treatment of patients with coronary artery disease with omega-3 PUFA, which can be used in practical medicine to correct the detection of changes and improve the effectiveness of treatment. Was first pointed out that the new plant substance leads to a modification of the fatty acid composition of erythrocyte membranes, liver and myocardium of rats, and inhibits the degradation of phospholipids and stabilizes the membrane. Analysis of the data shows that the modification of the fatty acid composition of cell membranes using a new substance of omega-3 polyunsaturated fatty acids of vegetable origin has a cardioprotective effect, which occurs due to inhibition of free radical reactions and increase the activity of antioxidant system.Obtained in this study have both theoretical and practical importance, as evidence of the possibility of modifications of the fatty acid composition of cell membranes with omega-3 PUFA both animal and, as we have shown, of new substance for the prevention and treatment of ischemic states