Dolzhkova K. NO-dependent mechanism of bone of mandible regeneration in the conditions of surplus income of sodium nitrate in the organism.

The investigation object: mechanisms of reparative regeneration of bone tissue of mandible. The investigation goal: the role of NO-synthases systems and peroxynitrite in reparative regeneration of bone tissue of a mandible after modeling of its fracture against a chronic intoxication of sodium nitrate. Methods of research: experimental, biochemical, morphological and tensiometrical methods of research of a mandible of white rats. Theoretical and practical value of the obtained results. The received results can be used for working out of pathogenetically well-founded methods of correction of processes reparative regenerationof a mandible at patients from districts with the surplus income of sodium nitrate at organism agents of activity of NO-synthase, a L-arginine and peroxynitrite scavenger. Scientific novelty of the obtained results: The research shows, that the activity of NO-synthase essentially influences to the reparative regeneration of a mandible osteogenesis at modeling of its fracture at rats. Introduction of substrate of NO-syntheses reaction — L-arginine partially limits depolymerization process of glycosaminoglycans (reduces level of gexuronic acids) in bone tissue of mandible, essentially doesn't influence the catabolism of fuko- and sialoglycoprotein. It is established that the formation of peroxynitrite negatively influences to a reparative osteogenesis of a mandible, because the use L-selenomethionine (peroxynitrite scavenger) authentically limits disorganization of biopolmers of a connecting tissue of bones of a mandible (reduces levels of chondroitin sulfates, untied with fibers fucosa, Nacetylneuraminic acids and gexuronic acids) on early terms of reparative regeneration of a bone formation. Whereas, on the later terms after fracture’s modeling peroxynitrite-dependent disorganization of a connecting tissue is mainly connected with depolymerization of glycosaminoglycans. The degree of implementation: results of work it is introduced in educational process on chairs of a pathophysiology, surgical stomatology and maxillofacial surgery with plastic and reconstructive surgery of a head and neck of the Ukrainian medical stomatological academy, chair of therapy and chair pathological anatomy and a pathological physiology of the Poltava state agrarian academy.