Sidyakin A. Inducted morphogenesis in vitro and the accumulation of triterpene glycosides in the callus cultures of grape clematis (Clematis vitalba L.)

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0411U002234

Applicant for

Specialization

  • 03.00.20 - Біотехнологія

13-04-2011

Specialized Academic Board

Д 53.369.01

Essay

The work is devoted to research of features introduction of conditions in vitro, explants of C. vitalba (= Old Man's Beard, Traveller's Joy, Grape clematis ), its processes of callus induction and accumulation of triterpene glycosides in the callus culture. The dependence of accumulation of triterpene saponins in the calluses on the composition of culture media, physical properties of cultivation and cytological characteristics of cell populations was shown. Developed by the biotechnology scheme induction and selection of callus cultures of C. vitalba, allowing to obtain strains containing glycosides at the level of their concentration in intact plants were was shown.

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