Golembiovska S. Characteristics and biotechnical potential of Crt +-mutants of Streptomyces globisporus 1912

Dissertation to obtain the scientific degree of candidate of biological sciences in speciality 03.00.07 - microbiology. - Zabolotny Institute of Microbiology and Virology National Academy of Sciences of Ukraine, Kiev, 2011. Dissertation is devoted to the study of biological ability mutants of Streptomyces globisporus 1912 with the constitutive biosynthesis carotenoids in different kind of mediums. It was shown the morphological ability Crt+-mutants. It was determined the spontaneous and induced variability of carotenoid biosynthesis production in Crt+-mutants. The mutants was high rate of non-activity biosynthesis carotenoids formations (Сrt- - mutants) 10-2-10-1, except, the mutant 6 Crt. The his frequency spontaneous and induced variability have been 10-6-10-4. It were defined violet-pink Lcp+-variants of mutants 4Crt, RVCrt and R3Crt on the potato medium, which among the intensive violet-pink mutant - 4 Lcp was isolated. The hereditary variability of the 4 Lcp has been 10-6. All Сrt+-mutants have accumulated low rate of antibiotics of landomycine Е and D looks like the initial strain 1912. Сrt+ and Lcp+-mutants have had resistant to action of the ultraviolet light and the peroxide in comparison with the initial stain 1912. Carotenoids of Сrt+- and Lcp+-mutants have been identified by ТLH, HPLC and spectrophotometry methods. The Сrt+- mutants have synthesized 47% of the lycopene and 22% of the beta-carotene from other synthesized carotenoids. Lcp+-mutants have accumulated of the lycopene 80%. It was formed efficient mediums for cultivation the perspective producer of lycopene - strain 4 Lcp. Synthetic mediums Krasilnikova СРІ, Waksman and Gauze have been suitable for cultivation of the strain 4 Lcp (2,0-4,0 mg/l). The natural complete medium (soya bean with maize) has had advantages in accumulation of weight and biosynthesis lycopene by the mutant 4 Lcp (33,4mg/l).