Bogdanchikova O. The human blood platelets cryopreservation in multicomponent cryoprotective media

The object of research - the functional properties safety of human blood platelets. The aim of research is cryoprotective properties definition of multicomponent media containing cryoprotectants combinations of amides and polyols series, at the cryopreservation stages of human blood platelets. Research methods: hematological, physical-chemical, spectrophotometric, fluorometric, refraktometric, the method of light microscopy. Experimentally justified a new approach to cryopreservatives creation for platelets freezing used on cryoprotectants combination in cryoprotective media, which belonging to different series of chemical compounds - amides and polyols. Designed media based on a combination dimetilacetamide with cryoprotectants 1,2-propanediol, glycerol or oxyethyleted glycerol with polymerization degree n=5. Shown that the cryoprotectants combination in various options does not increase the cytotoxicity of these media for platelets during exposure. The possibility of platelets uncontrolled freezing with combined cryoprotective media in the vapor of liquid nitrogen at a temperature of -188…-193 C is determined. Experimentally developed platelets freezing regimen with irregular cooling rate (12-25 C/min), the supercooling absence and the plateau crystallization duration 0,75-2,125min. For the first time established a direct significant correlation between the platelet functional properties indices after cryopreservation and cryoprotective media dynamic viscosity.