Kornia T. In vitro biotechnology for the rapid creation of homozygous material of winter wheat with resistance to Fusarium graminearum.

The thesis is devoted morphogenesis of various explants of common wheat under the influence of culture filtrate F. graminearum and fusarium mycotoxins, which will improve methods for assessing in vitro resistance to Fusarium head blight of wheat samples and determine the conditions of the biotechnology for the express creation of homozygous wheat materials with resistance to this pathogen at a combination of haploid methods and breeding in vitro. Revealed that the optimal selective factor for the evaluation of in vitro resistance of the samples of wheat to the disease is the culture filtrate (CF) of F. graminearum low pathogenic strain - ab, which is obtained by culturing the fungus on Czapek media modified organic nitrogen - a mixture of amino acids. It was established that to create in the anther culture in vitro by androgenesis homozygous forms of wheat with resistance to Fusarium head blight is effective to use as donor material for hybrid wheat crosses: ? (sensitive form to the androgenesis) ? ? (resistant form of wheat to F. graminearum) and as well as a selective factor - modified CF low pathogenic strain ab at a 5% concentration. It was established that the assessment in vitro resistance of samples of wheat corresponds assessment in vivo on 97% (rs = 0,97, p <0,05) by culturing 10 days mature embryos of wheat in the presence of a modified CF strain ab at a 15% concentration with the assessment of resistance by index of relations mass to the total length of the plants relative to control samples.