Klymenko N. Interaction of aminosugars with the surface of ultrafine oxides modified by albumin and properties of nanocomposites on their basis

Processes that occur in interaction of biomolecules (albumin and aminosugars) with the surface of ultrafine silica, titania/silica and alumina/silica have been investigated. The maximal adsorption of bovine serum albumin was observed on the surface of titania/silica. Adsorption of aminosugars N-acetyl-D-glucosamine and D-galactosamine depending upon the nature of adsorbent surface was investigated. The maximal adsorption of aminosugars was observed on the surface of mixed oxides. Adsorption parameters of obtained nanocomposites were calculated. It was shown by method of IR-spectroscopy that molecules of protein on the surface of ultrafine oxides transformed from coagulated globule to the unwrapped form which fact was confirmed by adsorption data. It was discovered that the peak of hydrogen sulfide evolution in temperature-programmed desorption mass spectrometry studies can be a test one for protein-covered nanocomposites. Intensity of hydrogen sulphide evolution was found to be proportional to the quantity of protein on the carrier surface. The change of water layer structure caused by interaction with ultrafine oxides and albumin was observed. Various forms of water (weakly associated one and that forming clusters of different size and composition) were also observed, which can be caused localization of water molecules in spatially separated cavities, formed by structural elements of cells. An increase in concentration of intracellular water leads to an increase in the quantity of strongly bound water that testifies in favor of changes in the properties of intracellular space. It was shown, that aminosugar adsorption on the surface of ultrafine silica increases viability of deconserved bovine reproductive cells, whereas pretreatment of the surface by protein decrease their mobility.