Hussein K. Development analytical methods for poisonings of aprophene diagnostics

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0411U007471

Applicant for

Specialization

  • 15.00.02 - Фармацевтична хімія та фармакогнозія

25-11-2011

Specialized Academic Board

Д 26.613.04

Essay

The first attempt of comparing analysis method to isolate aprophene from biological substances methodics applied for forensic toxicology and show it effectively studied condition (type of solution, pH of environment) and offered more effective method for isolation of aprophene from biologically fluids, allowed to isolate upto 86% (from blood) and 98% (from urine) analyte. Studied many methods to separate protein from blood, suitable for HLPC analysis. Maximal availability of aprophene is found when using acetonitryle as protein sedimentation. Next step of purification of plasma from lipophilic substances by hexane provide getting more pure material, suitable for use in columns. Established system immune - chromatographic test "Cannabis" "Моrphine", "Аmphetemine", "Меthamphetamine", "Ecstazy", "Cocaine", "Benzodiazepines", "Buprenorphine", "Barbiturates", "Methadone", "Propoxiphene", "Phencyclidine" having selectivity which satisfied and not provide pseudopositive result when available in the urine aprophene. Established, international (TIAFT) and local systems for screening toxic substances realizing by thin layer chromotography method to identify aprophene is impossible. Have been offered selective system (hexan - ethanol (3:1) и hexan - ethanol (1:1) + 0,1 ml 10% ammonium solution per 10 ml of mobile phase) for identify aprophene by thin layer chromotography method while available other illicit drugs. Pretreatment gas-liquid chromatography method to identification and determination of aprophene in biological material. Established that offered methodic have not systematic mistake and it is linearity from 0,5 to 50 mg/l. Pretreatment methods have been used to study the process of isolation of aprophene from biological material, effect of different deproteinisation by extractions them from blood, have been studied preserved in decomposed biological material. Established, that while preserving the period of 3 months contents of aprophene in the biological material decrees 80 %. Have been studied chromatographic character of aprophene and condition microclumn HLPC - analyzator and shows possibility of identify aprophene contains above drugs. The algorithm of design of research is made for forensic and clinical toxicology to identify and determine aprophene in biological materials or liquids of human organism.

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