Zinchenko T. Hygienic and toxicological aspects of prevention adverse effects of silver nanoparticles

Object of study: silver nanoparticles adverse effects on the worker's body during the nanosilver electron-beam synthesis. Purpose: hygienic assessment of operators working conditions, the toxic action definition of nanosilver on the body and the scientific rationale for negative impact of nanoscale silver prevention on the workers health. Research methods: hygiene, electron microscopic, histological, biochemical and quantum chemical and statistical. Theoretical and practical results: science-based prevention activities to optimize the operators working conditions. Developed method for determining the silver nanoparticles іn the air and methods of predicting the silver nanoparticles negative impact on the body. Novelty: the thesis is devoted to hygienic and toxicological assessment of new human factors nanosilver, which are synthesized by electron-beam technology and prevention aspects of adverse effects. It was given the hygienic assessment working conditions of operators who produce silver nanoparticles electron-beam technology. Harmful working environment factors were found when metals nanoparticles were getting electron-beam technology. We have shown that the dust aerosol of sodium chloride with silver nanoparticles is a novel specific factor for this production. Silver nanoparticles have been identified in the working area and studied their morphology. Morphological effects of vital organs have been established as result action nanosilver. It was found features ultramicroscopic respiratory changes of the test animal's lungs. It was received new scientific data, which elucidate molecular mechanisms of nanosilver action on the body. For the first time we've learned the specifics of the interaction silver nanoparticles from plasma membrane to the atomic-molecular level. Priorities for conservation measures and health promotion were grounded for employees of electron beam technology. The results of the work used in the preparation of the newsletter "The method predicting the nanosilver negative impact on the body" and introduced into the educational process at the university. Scope (area) use: in the institutions of the sanitary-epidemiological service and specialized research institutes.