Bayer O. Producing of flax (Linum usitatissimum L.) transgenic lines with dinitroaniline resistance

The current thesis is dedicated to the study of issues related to flax introduction in vitro as well as its transformation, selection, regeneration and rooting of transgenic shoots. As a result of flax genetic transformation using Eleusine indica (L.) Gaertn-derived mutant ?-tubulin gene, transgenic lines of Mogilevsky 2 variety have been obtained, characterized by the resistance to dinitroaniline herbicides. The selection scheme in the presence of trifluraline basing on the use of mutant ?-tubulin gene from E. indica as a selective marker gene of plant origin that confers the dinitroaniline herbicides resistance,. Molecular analysis confirmed the genetic modification of the obtained lines. It was established that the mutant ?-tubulin gene from E. indica has been effectively integrated into all types the arrays of microtubules in plant cells, insuring the higher resistance to the herbicides.