Lystvan K. The use of plants of tribe Psoraleeae (Fabaceae) for obtaining of biologically active meroterpene bakuchiol in the in vitro system

In the thesis the possibility of an obtaining of biologically active meroterpene bakuchiol by biotechnological means has been investigated. The feasibility of using of the species of tribe Psoraleeae as a basis for creation of different types of in vitro cultures has been studied. Aseptic plants for some species of the tribe Psoraleeae have been obtained and the bakuchiol content in them has been studied. The comparative study of bakuchiol content in the plants of different families has been carried out. The suitability of plants of tribe Psoraleeae as a source of bakuchiol has been demonstrated. They can accumulate bakuchiol in significant quantities, unlike plant species of other tribes (particularly, genus Plumbago, Spiraea and Piper). It has been shown, using one of the species of tribe Psoraleeae (Cullen drupaceum, synonym Psoralea drupacea), that cultures of dedifferentiated cells as well as cultures of isolated and transgenic roots failed to accumulate bakuchiol in any significant amounts. Methyl jasmonate and different growth regulators treatments failed to intensify the meroterpene biosynthesis. Aseptically-grown shoots, however, were found to accumulate up to 11% dry weight of bakuchiol and can therefore be regarded as a potentially useful source of this antimicrobial compound.