Heneha A. The influence of amino acids derivatives of 1,4-naphthоquinone on membrane related processes during loach embryogenesis

The thesis is intended to study membrane related processes of loach embryo Misgurnus fossilis L. under the effect of amino acid derivatives of 1,4-naphthoquinone. There have been shown dose-dependent inhibition of embryos of Na+, K+-ATPase amino acid derivatives of 1,4-naphthoquinone. It was revealed that the degree of inhibition impact depends on the stage of embryo development, the structure of corresponding amino acids derivatives of 1,4-naphthoquinone and the sensitivity of Na+, K+-ATPase to the action of the examined substances. There were defined constants of half-inhibition I50 for amino acids derivatives of 1,4-naphthoquinone. The analysis in Dixon coordinates showed the conditionally competitive type of inhibition. The results of ATPase inhibition confirmed changes of the TMP, the ultrastructure and the morphology of blastomeres of embryos. There were found such organelle damages in high concentration 10-6 M: oedema of SER channels, the increasing of lysosomes and secondary lysosomes number, as well as vacuoles, the destruction of plasma membrane, that leads to the metabolic disorders.