Karpenko O. Development of the biotechnology of complex remediation of soils, contaminated with oil and chlorine-organic substances

The thesis is devoted to the development of complex technological approaches to remediation of soil contaminated with petroleum and persistent chlorinated substances with the use of microbial preparations based on autochthonous microorganisms isolated from contaminated objects, biosurfactants, argillaceous mineral glauconite and chemical oxidants. The stimulating effects of various biogenic surfactants (rhamnolipids, trehalose lipids etc.) and chemical oxygen donors (Н2О2, СаО2) on the functional activity of microorganisms, particularly on the utilization of hydrocarbons and enzymatic activity, which determines the effectiveness of microbial treatment of soils, was ascertained. The effectiveness and synergistic effect of the use of agents with different mechanisms of action (microorganisms-destructors, biosurfactants, chemical oxidants in different compositions) in remediation processes when using them in one technology was established for the first time. The methods for soil remediation of toxic organochlorides, including PCBs and DDT, using oxidants (Н2О2, СаО2, Na2S2O8), as well as in combination with biosurfactants and microbial agents were proposed. The developed biotechnology scheme was tested in the field experiments (NGDU "Dolynanaftogas"): the effectiveness of the complex remediation approaches to reclamation of technologically contaminated objects was shown - the degree of degradation of contaminants in soil made 80-83%. A principal technology of the production of microbial preparation RVD1 for soil remediation was elaborated. These results create prospects of a practical use of the developed technologies of the remediation of contaminated sites and their overall recovery.