Kovalenko A. Participation of NO-synthases and peroxynitrite in pathogenesis of experimental traumatic sialadenitis

The investigation object: the role of of NO-synthases and peroxynitrite in pathogenesis of experimental traumatic sialadenitis (TS). The investigation goal: determining the role NO-synthase isoforms and their substrate L-arginine and peroxynitrite in the mechanisms of free radical processes activation and the development of bioenergy deficiency in submandibular salivary glands’ tissues under conditions of TS. Methods of research: experimental, biochemical, morphological. The research has shown NO-synthase activity substantially affects the superoxide anion radical production (.О), lipid peroxidation, bioenergetic processes in submandibular salivary gland tissue under TS. .О hyperproduction is connected with the functioning of inducible NO-synthase (iNOS) and is limited in neuronal NO-synthase (nNOS) functioning. It has been found out that under TS modeling iNOS functioning reduces ?-amylase and ornithine decarboxylase in submandibular glands. It is shown that the peroxynitrite scavenger L-selenomethionine administration significantly restricts metabolic and morphological alterations in submandibular glands and contributes to their secretory activity under TS. The degree of implementation: patent, practical innovation.