Kyselov D. Molecular-genetic analysis of breeding-valuable genotypes of apple (Malus domestika Borkh.

3.Modern molecular genetics investigations aimed at resolving the apple of theoretical and applied problems of introduction, preservation, restoration, identification, registration and certification of genetic resources. There was investigated the level of informative of IRAP- markers, derived from the used of primers to conserved areas of retrotransposons different genetic origin - apple Malus TRIM and soybean SIRE-1. Established that used markers were characterized by high information content, but retrotransposons Malus TRIM has higher discrimination ability.There was analyzed the genomic variability of cultivars and hybrid forms of apple used RAPD-PCR, developed a system of certification of apple cultivars from different genetic origin. By used IRAP 38 and 72 RAPD-markers were studied characteristics of genetic differentiation cultivars. For the first time the comparative analysis of the informative level of IRAP- and RAPD-markers, spent consensus analysis of these types of marker systems, clarified phylogenetics relationships between the studied genotypes of apple were done.The method of estimation of allele of scab gene resistance Vf was developed. For the first time screening of Ukrainian cultivars and allelic status of the gene Vf were determined. Unique dominant homozygote by Vf gene cultivar Interprais was revealed that should be used in breeding work. The method detection dominant allele Co of columnar habit of crown apple was developed. For the first time used molecular markers were performed a comprehensive analysis of Ukrainian and foreign cultivars of apple with gene Co. Key words: apple, molecular markers, IRAP, RAPD, scab resistance gene Vf, gene of columnar habit of crown Co.