Kharkhota M. Symbiotic composition on the basis of probiotic strains of Bacillus subtilis

The object of the dissertation is was to creation of sinbiotic composition on the basis of probiotic strains of B. subtilis UKM V-5139 and B. subtilis UKM V-5140, lactitol and lactulose and studying of their efficiency at experimental dysbacteriosis It is shown that the highest level of stimulation of growth of probiotic strains was observed with 15-20% of the content of the prebiotic in the cultivation medium. At the same time their adhesive properties have not changed. It is established, that a probiotic strains of bacilli are showing a high hydrolytic activity on polysaccharide and fatty acid. Growing at presents of cellulosic substrates strains were characterized by the CX -, Cl -, C2 and celobiases activities with temperature and pH optimum at 40 C and 6,0-7,0, respectively. For the actions of lactulose the cellulosolitic activity of probiotic strains grew in 1,5-3,8 times. It is shown that in the presence of prebiotics in the cultivation medium lytic activity at cells of S. aureus and P. aeruginosa, E. coli, C. albicans by bacilli were significantly highest (4-15%). It is proved that the most effective antagonistic activity is composition, containing probiotic strain of B. subtilis in a concentration of 10 lg CFU/ml at a ratio of 1:1 each, 15% of one of prebiotics and saccharose-gelatin composite. In experiments in vivo is high efficiency of the created symbiotic composition during the restoration of microbiocenosis of intestines of mice with experimental dysbiosis. Found immunomodulation action symbiotic composition, aimed at induction of endogenous interferonogenesis of macrophages peritoneal exudate.