Sosimchyk I. Rat liver state after hypothermic storage in the presence of mitochondria targeted agents

Object of research: energy, pro-oxidant-antioxidant and morphological state and secretory function of rat liver after hypothermic storage and normothermic reperfusion. The aim of research was to study the effect of preservation solution modifications with biologically active substances on energy, pro-oxidant-antioxidant, morphological state and secretory function of isolated rat liver after hypothermic storage and normothermic reperfusion. Methods of research - polarographic method for estimation of mitochondrial respiratory activity,spectrofluorimetric and spectrophotometric methods, histological evaluation of liver morphology and statistical processing of results. It was shown that bencyclane fumarate due to its uncoupling properties positively affects pro-oxidant-antioxidant balance in the liver at the stage of cold storage. Irreversible inhibition of mitochondrial functional activity after reperfusion attenuates this effect. It was shown that the supplementation of preservation solution with mitochondrial antioxidant SkQ1 results in improvement of liver pro-oxidant-antioxidant balance and mitochondrial function leading to partial prevention of ATP level decrease and normalization of organ morphological state and secretory function. For the first time the potential of fetal tissue cytosol application as a component of storage solution was demonstrated. It promotes the increase of mitochondria functional activity, positively affects the pro-oxidant-antioxidant and morphological state and secretory function of liver after long-term storage and subsequent reperfusion.