Porozhan I. Application of cryopreserved fetal neural cells to correct state of thymus during experimental allergic encephalomyelitis

The research object : structural and functional characteristics of animals thymus with experimental allergic encephalomyelitis before and after the introduction of cryopreserved fetal neural cells. The research aim was to study the characteristics of structural and functional changes in the thymus in the development of experimental allergic encephalomyelitis and the possibility to apply cryopreserved fetal neural cells for their correction. Research methods were cryobiological, cytomorphological, immunological, microscopic, biochemical,cytofluorimetrical, cultivation in the system in vitro, standard statistical methods. It was shown in comparative perspective the influence of different modes of cryopreservation on the structural and functional characteristics of fetal neural cells. A cryopreservation mode of fetal neural cells, which use by the combination of investigated indicators provides the most pronounced effect of "restoration" of mediator-producing, limphopoetic, the export function of the thymus and restoration of the natural tolerance of the organism. New data on significant differences of desensitizing effect of fetal neural cells cryopreserved by different regimens regarding antigen-reactive immune cells.  Information on the structural and functional changes of the thymus state in the development of experimental allergic encephalomyelitis can be taken into account to optimize therapies of neurodegenerative diseases of autoimmune genesis.