Umanskiy D. Forensic-medical person's identification with molecular-genetic methods of genomic DNA research, extracted from nuclei-containing cells in cytological specimens

The thesis is devoted to questions of person's identification in forensic medicine on the basis of PCR-analysis of genomic DNA of nuclei-containing cells in cytological specimens. Developed new method of nuclei-containing cells' extraction from the microtrace of biological origin increases the amount of extracted cells on 51,95±2,5 % in comparison with standard methods. New developed method of DNA extraction from nuclei-containing cells in cytological specimens, decreases the loss of nuclei-containing cells on 30,8 ± 0,9 % in comparison with standard methods. It was proved, that sweat-sebaceous microtraces contain the nuclei-containing cells, which could be examined using molecular-genetic methods for the purposes of person's identification. Cytological specimens with nuclei-containing blood cells are the most researchable groups with molecular-genetic methods among other specimens, produced from biological microtraces. Minimal amount of nuclei-containing cells, from which it is possible to receive DNA profile using developed algorithm of complex forensic-medical research, are as follows: 50 nuclei-containing blood cells, 46 epithelial cells, 48 cells of buccal epithelium, 48 spermatozoas and nuclei-containing sperm cells, 53 cells of vaginal epithelium.