Kovalenko G. Membrane Permeability of Rat's and Rabbit's Erythrocytes for Cryoprotectants

The object of investigation - transmembrane transfer of cryoprotectants belonging to different classes into rat and rabbit erythrocytes. The aim of the study, the permeability of the membranes of red blood cells of rats and rabbits for different classes of cryoprotectants at 37 and 25°C and establish ways of transmembrane transport processes.The methods of investigation - method of small angle of light scattering, light microscopy, methods of statistical processing, correlation analysis. For the first time the coefficients of rat and rabbit erythrocyte plasma membrane permeability to cryoprotectants belonging to diols and amides, multyatomic alcohols and their metoxy derivatives as well as to DMSO have been determined, and the values of their molecules transmembrane transport activation energy have been calculated. For the first time the mechanisms of rat and rabbit erythrocyte plasma membrane permeability to cryoprotectants of different classes of chemical substances have been established, and the cryoprotective acivity of these substances has been estimated. Modification of alcohols by exchange of hydroxyl (-OH) group for metoxy-group (СН3О-) leads to a substantial increase in the permeation rates of these substances across erythrocyte membranes, which may be due to change of their transport pathway. It has been established that the investigated substances permeability depends not only on their chemical structure and physico-chemical properties but also on species differences between rat and rabbit erythrocyte plasma membranes.