Shilin S. Phylogenetic analysis, identification and reclassification of methane- and methylotrophic bacteria

The dissertation considers identification questions of methane- and methylotrophic bacteria. Eight strains of obligate methane-oxidizing and 15 strains of facultative methylotrophic bacteria including 2 strains of methylotrophs isolated from extreme environment of Antarctica were identified and reclassified by using 16S rRNA sequence analysis. For the first time an independent taxonomic status of strain UCM B-3075 was established and a species Methylomonas rubra was described. On the basis of polyphasic analysis the thermotolerant strains of methane-oxidizing bacteria UCM B-3026, UCM B-3032 and UCM D-3109 were reclassified as Methylocaldum szegediense, and strain UCM B-3014 - as Methylocaldum gracile; a strain of mesophilic methane-oxidizing bacteria - UCM B-3159 - as Methylobacter marinus. For the first time the taxonomic status of the strains UCM B-3002 and UCM B-3494 was confirmed, both are carried to Methylococcus capsulatus species. Investigated facultative methylotrophs have been carried to the genus Methylobacterium by the study of complex of diagnostic features and have been identified. As a result strains of UCM В-3383 and UCM В-3357 are carried to the Methylobacterium mesophilicum species; UCM B-3360, UCM B-3362, UCM B-3368 and UCM B-3381 are carried to Methylobacterium extorquens; UCM B-3389 - Methylobacterium organophilum. For the first time were reclassified strains of facultative methylotrophs: UCM B-3339 as Methylobacterium zatmanii (previously was M. organophilum), UCM B-3342 as Methylobacterium fujisawaense (previously was M. organophilum), and UCM B-3351 as Methylobacterium fujisawaense (previously was M. extorquens). By results of the polyphase analysis for the first time it is offered the facultative methylotrophs M. extorquens, M. zatmanii and M. rhodesianum to unite into one species Methylobacterium extorquens; and facultative methylotrophs M. fujisawaense, M. radiotolerans and M. mesophilicum into one species Methylobacterium mesophilicum.