Gudzenko O. Alfa-L-Rhamnosidases of Cryptococcus albidus and Eupenicillium erubescens

The thesis for a candidate's degree by speciality 03.00.07 - microbiology. - Institute of microbiology and virology named D.K. Zabolotny of National Academy of Sciences of Ukraine, Kiev, 2013. The dissertation is focused on investigation of Cryptococcus albidus 1001 and Eupenicillium erubescens 248 alfa-L-rhamnosidases activity. The conditions and parameters of cultivation of alfa-L-rhamnosidases producers have been optimized. The scheme of enzyme purification have been selected. From C. albidus and E. erubescens culture supernatant liquids preparation of purified -L-rhamnosidases with specific activity 12,5 and 120 U/mg protein, respectively have been obtained. It was detected, that the investigated enzymes exhibited narrow specificity with respect to the synthetic derivatives of monosaccharides and efficiently hydrolyze natural substrates.