Horchyn S. Biotechnological methods for improving of sperm quality of boars for short-term storage

Object is the physiological and biochemical processes in the semen of boars under conditions of dilution and storage of refrigerated. The goal is to find out effectiveness of application of the combinations of antibiotics, water extract of propolis and hydrophilic extract of oak silkworm pupae during the short-term storage of semen of boars in the refrigerated state and develop biotechnological methods to improve sperm quality. Methods: biotechnology (drug development, the environment, artificial insemination), physiological (motility, sperm concentration), biochemical (determination of protein, carbohydrate and lipid metabolism in sperm), kinetic (computer analysis of sperm motility) and statistical (biometric processing of results). Novelty: On the basis of comprehensive research of the study of the microbial contamination of semen of boars, determination of the optimal doses of antimicrobial drugs and biologically active substances developed biotechnological methods in order to improve sperm quality. For the first time the peculiarities of metabolism in the semen of boars were determined - oxidative and hydrolytic enzymes, lipid peroxidation (LPO) and the antioxidant defense system by use of complex antibiotics, water extract of propolis and hydrophilic extracts of silkworm pupae oak. It was determined that during the use of investigated antibiotics and biologically active substances in boar semen diluent, the intensity of the release of enzymes and other components of cellular metabolism decreases, that indicates a decrease in sperm damage. It was investigated that first kinetic parameters of boar spermatozoa while using computerized system CASA (Computer Assisted Semen Analysis) which confirm the complex (antimicrobial, antioxidant, anti-toxic, stimulating) effects of antibiotics, water extract of propolis and hydrophilic extracts of silkworm pupae of the oak and make it possible to predict the impregnating ability of sperm. Results: A comprehensive antimicrobial agent for sanitation and environment boar semen to liquefy and short-term storage of semen of boars' Ekosperm B " was developed with the usage of a new antibacterial agent (Patent for useful model № 84411 from 07.10.2013 PU). The efficiency of water extract of propolis and its optimal dose was determined with high antimicrobial activity. A method of applying an aqueous extract of oak silkworm pupae by dilution and storage of semen of boars was developed investigated.