Shablii V. Characteristic of multipotent mesenchymal stromal cells derived from native and cryopreserved human placental tissue

The research is dedicated to investigation of immunophenotype, differentiation potential and functional potency in vivo of multipotent mesenchymal stromal cells (MMSC) of fetal origin isolated from native and cryopreserved human placental tissue. The method of isolation MMSC from cryopreserved human placental tissue has been developed. It was shown the similarities of molecular and biological properties of MMSC isolated from native and cryopreserved human placenta. It was firstly studied that placental-derived MMSC of fetal origin simultaneous expressed of mesenchymal (vimentin) and trophoblast (cytokeratin AE1/AE3, cytokeratin 7, chorionic gonadotropin, epidermal growth factor receptor 2 HER2) markers. Also we firstly detected expression genes PPAR-?2, COL2A1 and SPP1 in culture of MMSC from both native and cryopreserved human placenta. MMSC isolated from cryopreserved fetal tissue and term human placenta had the same morphology, immunophenotype and ability to multipotent differentiation with MMSC from native tissue. It is shown that MMSC isolated from native and cryopreserved mature placenta were able to survive, to migrate and to integrate into the damaged cardiac tissue in vivo after intravenous transplantation. MMSC isolated from native and cryopreserved term placenta positive impact on the regenerative processes in the myocardium of mice with isoproterenol-induced cardiomyopathy. We observed increasing in metabolic activity of individual cardiomyocytes (hypertrophy of nuclei), the weakening of the inflammation process and normalization of electrophysiological excitability of myocardium, but sometimes it was found increased synthesis of connective tissue elements.