Magorivska I. Antigenic specificity and proteolytic activity anti-histone antibodies and their effects on proliferation and viability of tumor cells

Object of the research: human cancer cell response on the presence of the anti-histone IgG from blood serum of patients with systemic lupus erythematosus and multiple myeloma.The aim of the work: determine antigenic specificity and catalytic activity anti-histone antibodies purified from serum of patients with systemic lupus erythematosus and multiple myeloma, and describe their impact on the viability of tumor cells in vitro.Methods: cell-biology, biochemical, immunological, mathematical statistics. For the first anti-histone H1 IgG has been purified from the blood serum of patients with systemic lupus erythematosus (SLE ) and multiple myeloma (MM). For the first time found that anti-histone H1 IgG stimulate proliferation of human acute lymphoblastic leukemia cells line CEM in vitro. For the first time found that anti-histone H1 IgG possess proteolytic activity against histone H1 and myelin basic protein. For the first time shown that oligosaccharide residues anti-histone IgG is less sialylated compared to the overall pool of IgG, which was purified from the blood serum of patients with SLE. Nonsialylated IgG fraction of serum of patients with SLE have a higher affinity to total histone fraction compared to sialylated IgG. Area of use: cell biology, immunology, molecular biology.