Starosyla D. Properties of novel subsances based on plant flavonoids and mechanisms of their antiviral activity

The thesis deals with the analysis of the properties and mechanisms of antiviral activity of 7'3'-dimetoxy quercetine (DMQ) as the principal component of Proteflazid plant-derived composition and the minor synthetic component 5,7,3,4'-tetrametoxy quercetine (TMQ). The antiviral activity of the substances under study was assessed in vitro and in vivo in modeled experimental virus infections, namely influenza, herpes types 1, 2, hepatitis C. DMQ and TMQ used both in prophylactic and therapeutic schedules inhibited effectively such generalized herpetic infection. Novel data on the anti-influenza mechanisms of DMQ related to neuraminidase inhibition have been provided. For the first time, DMQ as flavonoid component of Proteflazid has been shown to induce interferon (IFN) and up-regulate protein kinase (PK), 2'5'-oligoadenylate synthase (2'5' OAS) and RNAse L expression with relative decrease of 2'5' OAS up-regulation. The method for classifying IFN inducers according to the relative expression of PK, 2'5' OAS and RNAse L genes has been elaborated and patented.