Gojster O. Development of express biotest methods for detecting T-2 toxin

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0414U001766

Applicant for

Specialization

  • 03.00.20 - Біотехнологія

28-04-2014

Specialized Academic Board

Д 26.240.01

Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine

Essay

Object: biosensors for detection of T-2 toxin. Aim: Development methods for detection of T-2 toxin using opticals biosensors based on specifical antibodies and calixarenes and analytical biotests on the basis of Daphnia magna’s living medium chemiluminescence and bacterial bioluminescence. Methods: surface plasmon resonance, total internal reflection ellipsometry, chemiluminescence, bioluminescence, immunoenzyme and statistical analysis. Development of express instrumental analytical biotests for detection of T-2 toxin by detection of Daphnia magna’s living medium chemiluminescence und bacterial bioluminescence, as well as the immune biosensors for detection of T-2 toxin in solutions, based on the surface plasmon resonance and the total Internal reflection ellipsometry principles.

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