Voitovich O. Features of microbiocenosis of the mucous membrane of the nose of the human in the conditions of industrial city

Object of study: microbiocenosis human nasal mucosa. Objective: to determine peculiarities of the microbiota of the human nasal mucosa under conditions of ambient air pollution xenobiotics industrial city (for example, Zaporizhzhia) by examining the species composition of the microbiota and its biological properties, performance rhinocytogramm and local innate immunity. Research Methods and apparatus: microbiological definition of environmental performance, adhesive and antimicrobial activity, cytological, ELISA, immunocytochemical methods using microscope PrimoStar, ELISA analyzer SIRIS, digital image analysis system AxioVision, computer applications "Statistica 5.0" (StatSoftt Inc ., USA), "ApiWeb" (BioMerieu, France), digital image analysis ImageJ (NIH, USA) WHONet -5,5 (WHO, 2001). In the nasal mucosa residents of contaminated areas xenobiotics Zaporozhye established order of magnitude greater intensity of colonization of coagulase-positive staphylococci, gain a dominant role for Candida albicans. For the first time shown that more than 50% of strains of coagulase-negative staphylococci isolated from nasal mucosa inhabitants of Zaporozhye is oxatcylin resistance and first established the prevalence of mecA phenotype among coagulase-negative (29.4% of strains) and coagulase-positive (16.7% of strains) staphylococci. The dependence between expression of TLR 2 diabetes, TRL 4-type immune proteasome subunit LMP2 and NF-k B in epithelial cells and neutrophils nasal mucosa, the species composition of the dominant microbiota factors and man-made pollution. Improved methods for evaluating adhesive activity and methods of isolation penitsylinorezystentnyh strains of pneumococci. Area of use: understanding the role of Toll-like receptors in the functioning of the microbiota will use them primarily as a "target" of action of probiotics in conditions of dysbiosis. Scientific results obtained in the thesis are introduced into practice of health care, are used in the learning process and can be applied in microbiology laboratories, infection, therapeutic units residential health care facilities.