Falko O. Influence of low temperature preservation on antiatherogenic effect of placental blood serum

Research object was the effect of low temperature storage on protein peculiarities and antiatherogenic effect of cryopreserved human placental blood serum (cHPBS). Research was aimed to study the effect of storage at -20 and -196°C on HPBS proteins and the action of cHPBS on animal organism in experimental atherosclerosis. Research methods were as follows: electrophoresis in PAAG, gel-chromatography, spectrophotometry, fluorescent spectroscopy, method of fluorescent probes, atherosclerosis modelling, morphometric, planimetric, biochemical and histologic methods, light microscopy, methods for statistical processing of findings. Results: proteins as a component of human placental blood serum do not create a spontaneous aggregation during its freezing and long-term storage at -20 and -196°C, but due to namely freeze-thawing down to the mentioned temperatures an increase in a number of diamide-induced aggregate protein complexes occurs. The storage of human placental blood serum within 6 and 12 months at -20 and -196°C respectively provides the integrity of its proteins content. Storing human placental blood serum at -20°C within 6 months causes the augmentation of fluorescence intensity by 15%, but the storage term prolongation up to 12 months leads to a decreased intensity of own fluorescence of human placental blood serum proteins and an increased fluorescence intensity of DCP4-6 probe after its interaction with serum proteins. Freezing of placental blood serum down to -196°C causes an increased intensity of protein fluorescence by 15% without changes during following storage at the mentioned temperature. The introduction of cryopreserved human placental blood serum into experimental animals with modelled atherosclerosis causes a sanogenetic effect on animal organism, which was not different from the action of native human placental blood serum.