Bunduk Y. Оптимизация биотехнологических этапов клонального микроразмножения форм айвы продолговатой (Cydonia oblonga Mill)

In the dissertation work the experimental and theoretic material is firstly set out concerning the problems of biotechnologic devices optimization for quince (Cydonia oblonga Mill.) sanitation and clonal micropropagation. The conditions for thermo- and chemotherapy іn vitro are determined, which give possibility to provide a higher reliability level and a guaranteed yield of quince non-viral material. The optimum terms of outgoing material selection and plant material sterilization conditions with the aim of obtaining an aseptic culture are clarified. The most optimum growth regulators concentrations are selected, particularly ?-indolil butyric acid, indolil-3-acetic acid, naftilacetic acid, benzylaminopurine, kinetin, gibberellic acid, mineral and carbohydrate components of nutrient medium for morphogenesis different stages, the usage of which allows to increase significantly the effectiveness of quince clonal micropropagation process. In conditions of various spectrum content light effect on quince microshoots development at proliferation stage, a physiologic reaction of different types is detected on a certain spectrum lamps illumination. It is shown that the lamps illumination with radiation in a red spectrum site causes the optimization of quince plants biometric indicators. The microshoots іn vitro rhizogenesis stage is improved (a short-time culturing of microshoots in darkness, an optimum medium selection for rhizogenesis), which gives possibility to provide a high-level rooting formation and quince microshoots rhizogenesis process acceleration. The usage of luminescent lamps illumination with radiation enforcement in a blue spectrum site is offered first. This provides more quince rooted microshoots yield in conditions in vitro. The stage of quince regenerating plants acclimatization ex vitro is optimized first, which is based on using a two-layer substrate, stipulating their establishment level increase. A stimulating effect of boric acid- and potassium permanganate solutions is revealed on adaptation process of quince regenerating plants in glasshouse isolators. The biotechnological scheme of obtaining nonviral planting material of quince form is elaborated for propagation in culture in vitro.