Shchurska K. Substantiation of parameters of biotechnological processes of hydrogen production

The thesis is devoted to the development of biotechnological method for hydrogen production in bioelectrochemical system for different wastes utilization. The rational process parameters have been determined: anodіс biofilm with high exoelectrogenic activity, presented by the association of microorganisms; applied voltage 0.6 V; pH 6-7.5; sodium acetate concentration from 1 to 5 mM. The new three-stage method of biofilm formation with high exoelectrogenic ability on the anode of bioelectrochemical systems (BES) was developed and constructively executed. Stimulating effect of aspartic acid on the process of bioelectrochemical hydrogen production was found. The possibility of practical implementation of fundamentally new bioelectrochemical process as the basis of modern biotechnology for hydrogen production has been proved. Possibility of various organic wastes using as a nutrient medium for exoelectrogens in BES was substantiated: the efficiency of organic brewery wastewater compounds that had been pre-treated under anaerobic conditions with hydrogen producing bacteria was 0.02 g H2/g COD; the efficiency of glycerol conversion to hydrogen is 39 %; the efficiency of hydrogen production from malting plant wastewaters is 0.01 g H2/g COD. A technological scheme of malt plant wastewater treatment hydrogen production in bioelectrochemical systems was developed and its approbation was held on malt plant. The technology provides a gas mixture with 99 % of hydrogen with low energy costs and ensures the required quality of treated wastewater.