Dumych T. Mechanisms of changes in the plasma membrane during apoptosis and its role in intercellular interactions

Object of the research: glycoproteins of the plasma membrane cells during apoptosis. The aim of the work: to determine changes of the plasma membrane glycoproteins of mammalian cells during apoptosis and its influence on the processes of intercellular interactions. Methods: cell-biology, biochemical, immunological, mathematical statistics. For the first was demonstrated that apoptotic cells release distinct types of apoptotic blebs; one type is derived from endoplasmic reticulum, while other is derived from plasma membrane. "Immature" oligomannose glycans were proposed as a new diagnostic marker for the detection of dead cells, suitable for analysis in the whole blood samples. It was shown that adherent-invasive E. coli (AIEC) can stimulate an increase of mannose-containing glycans in the surface of dying cells and apoptotic vesicles and utilize them for further host cell colonization. It was found that addition of mannoside-based inhibitors prevents binding of AIEC towards host cells in bacterial-cells co-cultures at 500 nM concentration, being 5000 times lower than corresponding inhibitory concentration of natural inhibitors.Area of use: cell biology, immunology, molecular biology.