Rodionova V. Hygienic evaluation and regulation of modern protective chemical means of fruit crops

Research object: the behavior in the environmental and working environmental objects, toxicity and hazardous for workers, population and environment of Masai, Rehalis and Bellis and their active ingredients when used to protect apple trees in soil and climatic conditions of Ukraine. Methods: method of model and laboratory hygienic experiments, during which used the organoleptic, sanitary-chemical, sanitary and microbiological, chemical and analytical (high performance liquid chromatography and gas-liquid), physical and statistical methods, bibliographic method. Theoretical and practical results: toxicological and hygienic assessment of tebufenpyrad, prohexadion-calcium, pyraclostrobin and boscalid were conducted based on the literature; hygienic classification of the studied substances and pesticides was made on base of their danger degree; acceptable daily intakes, safe exposure levels in the air of the working area and atmosphere air, the maximum allowable concentrations in apples and apple juice of tebufenpyrad and prohexadion-calcium were justified; tentatively allowable concentration in soil, the maximum allowable concentration in water of tebufenpyrad and prohexadion-calcium were also justified; risk of adverse effects of studied substances for those who works with Masai, Regalis and Bellis were calculated. The optimal conditions for joint determination in a water sample tebufenpyrad, prohexadion-calcium, pyraclostrobin and boscalid, chromatographic separation of substances, detection and quantitative determination by high performance liquid chromatography were defined. Scientific novelty: comparative assessment of toxicity and metabolism of modern protective chemical means in the organism of warm-blooded animals and environmental objects were conducted for the first time. It allowed to justify acceptable daily intake of tebufenpyrad and prohexadion-calcium for human; professional risk of studied preparations influence on professional communities was under their usage for apple trees in soil and climatic conditions of Ukraine; peculiarities of behavior of these substances in agrocenosis objects of different soil and climate ones of Ukraine were determined and assessment of their ecotoxicological hazard was conducted. Level of inculcation: results are inculcated in patent on utility model and instructions for safe use of Masai, Regalis and Bellis conditions of their use to protect apple orchards. Sphere of application: hygiene, agriculture and medicine