Object of study: the causal relationships between human diseases, as commensal microbiota of the gut and immune status. Aim of the work was to identify key microbial and immune markers for the diagnosis of obesity, type 2 diabetes, metabolic syndrome and cardiovascular disease clarify the rights and immunomodulatory and anti-inflammatory properties of extracts of edible plants rich in biologically active substances. Methods: molecular microbiology techniques (MALDI mass spectrometry, NDS-PAGE electrophoresis), immunological (ELISA, flow cytometry), preclinical in vitro (fragments cultivation of cells and tissues) and in vivo. (Animal models), methods mathematical statistics (correlation and cluster analysis, variation statistics). yeoretychni and practical results: first proposed to use indicators gut microbiota and immune markers of subclinical inflammation unspecific for early diagnosis of cardiovascular disease (CVD), metabolic syndrome (MS), including obesity and diabetes mellitus type 2 (DM2). Extracts selected by us edible plants - dill (Anethum graveolens L.) , nettle (Urtica dioica L.), kale (Brassica oleracea var. Sabellica L), persimmon (Diospyros kaki Thund.), pomegranate (Punica granatum L.) and mursalskoho tea (syderitisu, Sideritis syriaca) - found to specifically alter the microbiota line the intestine of mice BALB / c and modulate the immune response locally in different compartments of the small intestine and structural separations associated intestinal lymphatic tisue; plant extracts of fennel, nettles, kale, persimmon, pomegranate and tea are able mursalskoho modulate the immune response, as evidenced roa study of cellular structure peyerovyh patches, mesenteric lymph nodes and other lymph kyshkovoasotsiyovanyh fabric by flow cytometry, by laser ion mass spectrometry MALDI, biochemical variability of microorganisms under the influence of plant extracts; reasonable hypothesis about the potential efficacy of personalized diets to prevent a number of chronic diseases associated with metabolic disorders and eating habits, received science-based database of experimental data on the use of food in the diets of different species of edible plants and the development of complex drugs targeting patients with different somatic pathology. Degree of implementation: three district hospitals.
