Demianenko I. Technology of detection and localization of biogenic magnetic nanoparticles and conditions of their formation

The thesis is devoted to the study of biomineralization process in prokaryotes and eukaryotes, as well as the development of technology to identify BMN in biological objects. We have defined the microorganisms that were potential producers of biogenic magnetic nanoparticles by bioinformatics methods (comparative genomics). It was found theoretically and experimentally that the process of biomineralization passes regardless of the presence or absence of ferritin in the prokaryotes and eukaryotes cells. We developed the technology of definition and localization of biogenic magnetic nanoparticles on the basis of magnetic force microscopy. Approbation of the technology was conducted at a magnetically operated biosorbents based on complexes of magnetic nanoparticles - yeast Saccharamyces cerevisiae obtained by mechanical and magnetohydrodynamic stirring with different exposures. The structural localization of biogenic magnetic phase in Ehrlich ascites carcinoma was defound using the developed technology. Theoretically justified and identified biogenic magnetic nanoparticles in samples of sections of atherosclerotic plaque mixed structure. Key words: biogenic magnetic nanoparticles, Ehrlich ascites carcinoma, atherosclerotic plaque, magnetically-driven biosorbents.