Savenko U. Genetic, immune, and clinical criteria for projection and optimization of allergic diseases prevention in children.

The thesis is devoted to a current issue of modern pediatrics - the study of clinical and paraclinical features of allergic pathology and its prevention. This thesis presents the results of studies of the glutathione-S-transferase group of genes: GSTT1, GSTP1, GSTM1 in 48 newborns and 49 children with asthma (55.1%) and atopic dermatitis (44.9%), 26 (54.2%) newborns were with aggravated atopic anamnesis. Molecular genetic determination of polymorphism of GST genes was measured by PCR. The results of the study demonstrate the relationship between the allelic polymorphism of glutathione-S-transferase genes in newborns and phenotypical realization of atopic dermatitis during the first year of life. The severity of the clinical course of allergic pathology is directly proportional to the allelic polymorphism in the The thesis is devoted to a current issue of modern pediatrics - the study of clinical and paraclinical features of allergic pathology and its prevention. This thesis presents the results of studies of the glutathione-S-transferase group of genes: GSTT1, GSTP1, GSTM1 in 48 newborns and 49 children with asthma (55.1%) and atopic dermatitis (44.9%), 26 (54.2%) newborns were with aggravated atopic anamnesis. Molecular genetic determination of polymorphism of GST genes was measured by PCR. The results of the study demonstrate the relationship between the allelic polymorphism of glutathione-S-transferase genes in newborns and phenotypical realization of atopic dermatitis during the first year of life. The severity of the clinical course of allergic pathology is directly proportional to the allelic polymorphism in the GST group of genes. The deletion polymorphism in GSTM1 gene was higher in children with atopic anamnesis 72.7%, as compared to children without atopic anamnesis 25% (p=0.006); 80% and 41.7% for allelic polymorphism GSTP1 A313G - AG GSTР1(p=0.006). We have discovered a relationship between mutations present in the GST group of genes and such disorders as asthma and atopic dermatitis. There is a directly proportional dependence between the severity of the clinical course of allergic pathology and the number of structural changes in the GST group of genes. Children with severe and moderate asthma had deletion polymorphism in GSTM1 gene in 85.7% cases and with mild asthma only in 15.3% of cases (p=0.0002). In children with severe clinical course of atopic dermatitis deletion polymorphism in GSTM1 gen was in 75% of cases and only in 25% of cases with mild clinical course of atopic dermatitis (p=0.004). Based on a comprehensive analysis of the clinical, genetic, and immune parameters, we have created an algorithm that allows predicting the development of atopic dermatitis during first year of life.