The dissertation is dedicated to research of phosphate mobilized and nitrogen-fixing bacteria physiological characteristics in their coordination with natural and synthetic disperse materials. Influence of natural and synthetic disperse materials on growth of Azotobacter vinelandii ІМV В-7076 and Bacillus subtilis ІМV В-7023 was investigated. It was showed that natural materials introduction into medium caused significant stimulating effect on growth of nitrogen-fixing bacteria. The amount of microorganisms was higher in 2 - 6 times in research variants, than in control, which depends on medium type and disperse material. However, in condition of titanium dioxide influence, regardless of growth-supporting micro medium contents, the amount of A. vinelandii was in 5 times higher, than in control, which correlates to data, received as a result of microelectrophoresis method in relation to component contents of azotobacter cell surface. It is found that stimulating influence of disperse materials on B. subtilis growth depends on phosphate contents in the medium. Thus, at concentration of РО43- in the amount of 0.6 g/l we can observe increase of bacteria growth capacity, comparing to control. On the contrary, natural disperse materials introduction into medium, in the condition of phosphate concentration in the amount of 0.1 g/l, caused decrease of this indicator. However, growth capacity stimulation of Strr-mutant B. subtilis with use of disperse particles takes place both at high and low phosphate concentrations in culture medium, which is caused by the type of experimental strains bacterial surface. It is found that the bacteria A. vinelandii and B. subtilis can come into contact interrelation with particulate materials. This is evidenced by the results of electron microscopy, decrease of ?-potential of cells at their interrelation with disperse particles. Influence of disperse materials on biosynthesis of biologically active substances by bacteria A. vinelandii IMV B-7076 and B. subtilis IMV B-7023 was researched. It is found that at adding azotobacter vermiculite to the medium, total concentration of abscisic acid increased in 2 times, and it increased in 1.5 times, in the condition of B. subtilis cultivation with this mineral, comparing control. At the same time, indole acetic acid concentration increased in 4.5 times in culture medium of bacilli. It is showed that at introduction of silicone dioxide into azotobacter medium, zeatin contents increased in 2.5 times and zeatin riboside contents increased in 3.5 times, comparing to control. Key words: Azotobacter vinelandii, Bacillus subtilis, growth, ?-potential, adhesion, glauconite, saponite, phosphorite, IAA, ABA.
