Biliavska L. The reproduction of viruses and the drugs antiviral activity under mixed adeno-herpetic infection

The thesis deals with the study of the reproduction of adeno- and herpesviruses in models of mixed infections and the peculiarities of the action of antiviral compounds under these conditions. For the first time the models of the mixed adeno-herpetic infections in epithelial and lymphoblastoid cell lines of the human and animals (Raji, Namalwa and MDBK) were created. The influence of the viral infection on morphological and functional properties of the cells was studied in these models. The inhibition of viability (40 %) and proliferative activity (82 %) of the co-infected lymphoblastoid cells (Raji and Namalwa) was observed. Shown that the 32 % reduction of the viability of the co-infected MDBK cells was due to the inhibition of the dehydrogenase activity of mitochondria. The changes of the structure of the nucleus and cytoplasm of the co-infected cells were found to be less pronounced in comparison to the monoinfection. For the first time the features of the reproduction of associated-viruses under conditions of the mixed infection were studied. The stimulation of the reproduction of adenovirus on 0.5 - 1.9 lg and 2 - 12 % inhibition of EBV reproduction were shown in both lymphoblastoid cell lines compared to the monoinfection. These effects depended on the multiplicity of adenovirus. The DNA replication of both viruses decreased on 6% and the virus titers reduced on 2.6 and 1.6 lg, correspondingly, compared to mono infection. Shown the simultaneous inhibition of protein synthesis of adeno- and herpesvirus on 17 and 83 %, respectively, under the MDBK cells co-іnfection. For the first time the models of mixed adeno-herpetic infection of cells were used to study the activity of some officinal antiviral drugs and abnormal nucleoside G8. The resistance of the viruses to the etiotropic drugs was shown under these conditions.