Melnyk O. The role of NО- and Na+-ATP-dependent regulatory systems in the reactive arthritis pathogenesis

Object - reactive arthritis; purpose - clarify the pathophysiological mechanisms of reactive arthritis involving arginase-NO-synthase, the transport ion-regulatory systems and cellular immunity; methods - immunological, biochemical, microbiological, spectroscopy, electron microscopy, statistical analysis; results - the most sensitive metabolic and immunological parameters of blood lymphocytes involved in the regulation of pathological processes are found; changes in the activity of endothelial and inducible isoforms of NO-synthase, arginase, Na+,K+-ATPase, H+-ATPase and content of stable nitric oxide metabolites in the peripheral blood lymphocytes under the conditions of this pathology are discovered; the decrease in eNO-synthase, an increase in iNO-synthase and arginase activities, increase in the concentration of NO - NO2- and NO3- metabolites, and decrease in Na+,K+- and H+-ATPase activities are established; it is shown that changes in the activity of the enzyme arginase-NO-synthase system is accompanied by interruption of the functional activity of phagocytic cells and change of T-lymphocytes subpopulations interrelations; the major infectious factors that mostly cause reactive arthritis developing, in particular chlamydia and ?-hemolytic streptococcus, are analyzed and identified; the search of new compounds - inhibitors of bacterial growth-triggers of reactive arthritis development is conducted and their inhibitory effect on the activity of both NO-synthase isoforms is set; among the studied enzymes potential additional markers of the reactive arthritis such as arginase and iNO-synthase are identified.