Guminska O. State of specific reactivity of immature rats on a background of drug-induced hepatitis

Object - chronic drug-induced hepatitis in immature rats; purpose - to find out the status of specific reactivity mechanisms of in-jury and regeneration of tissues of the thymus, liver, spleen in immature rats in conditions of chronic drug hepatitis and develop new approaches to pathogenetic correction of violations; methods - experimental, immunoassay, enzyme, tsytofluorymetrycal, mor-phological, mathemetic; results - for the first time there have been revealed the features of the immune system - the number of CD3+, CD4+, CD8+, CD22+ in blood of immature rats with chronic drug-induced hepatitis and after its correction by antral and quercetin; it is proved that secondary combined immunodeficiency accompanied by a significantly lower number of CD8+ and consequently higher immunoregulatory index occurs during chronic drug-induced hepatitis in immature rats; аlso is found that after using quercetin datas of immune system were as close to those of intact group of rats. In chronic drug-induced hepatitis reproduced in immature rats the level of profibrogenic cytokine – transforming growth factor-?1 in serum was higher, after using quercetin its level was significantly lower; there was revealed a smaller percentage of liver, thymus and spleen cells nuclei revealed in the cell cy-cle resting phase in immature rats with drug-induced chronic hepatitis; a higher proliferation index and index of nuclear DNA frag-mentation in liver, thymus and spleen cells were found. In chronic drug-induced hepatitis there were «cirrous» cells, infiltrates of var-ious shapes, foci of necrosis revealed in liver tissue, and decreased number of cells, "exhausted" areas and "starry sky" appearance – in thymus tissue.