Kuchma M. Comparative characteristic of hematopoietic stem/progenitor cells derived from human placental tissue, cord blood and fetal liver

The comparative analysis of HSPCs form placental tissue, cord blood and fetal liver was conducted. The methods of isolation of HSPCs from the placental tissue were modified. The method of isolation of HSPCs from cryopreserved placental tissue was developed. We have shown that hematopoietic progenitors were more heterogeneous by their phenotype in compare to the cord blood. The HPCs compartment of the placenta versus the cord blood contained higher number of committed cells but their lower number compared to the fetal liver. HSPCs of the placenta and the cord blood origin possess similar potentials to the multilineage differentiation in vitro. The new type of flat erythroid colony was detected and described by us. Our data suggests that the active hematopoiesis occurs in the placenta and it is an additional source of HSPCs for treatment of oncohematological diseases and congenital hematologic disorders. Keywords: hematopoietic stem and progenitor cells (HSPC), cell technologies, differentiation, hematopoiesis, placenta, umbilical cord blood, fetal liver.