Zyuzyun A. Cytomorphological features of the oocyte-cumulus complexes and embryos of farm animals when cultured in vitro

In the dissertation presents results of research cytomorphological characteristics OCC rabbits, pigs, goats and their maturation in conditions in vitro. Conducted analysis of fertilization and embryo development rabbits and pigs outside the body. Scientifically substantiated and experimentally demonstrated that source of oocytes is more expedient to use ovarian in follicular growth phase because of their ovaries can be more suitable to the cultivation of oocytes in vitro they have much less denaturated gametes. Obtained informations optimal time parameters maturation of oocytes in vitro studied species - rabbits, pigs and goats. In conditions in vitro maturation oocytes in rabbits continues - 24 hours, in the goats - 27 hours and pigs almost twice as long - 44 hours. Established, that obtaining rabbit embryos from oocytes in vitro, was provided, the maturation oocytes removed to metaphase II at 89 % and embryo development 68,1 %. Thus, the method does not concede the efficiency of the method of obtaining eggs matured in vivo using hormone stimulation.