Hrynchuk K. 3. "Molecular diagnostic and identification of beet necrotic yellow vein virus"

3. The monitoring of Ukrainian agrocoenosis has been made for Beet necrotic yellow vein virus (BNYVV) searching, which was used as a positive control for the development of diagnostics based on polymerase chain reaction (PCR). ELISA method has established the presence of protein in the samples of sugar beet. Using bioassays and electron microscopy has confirmed the presence of BNYVV. Polyclonal antibodies for BNYVV identification have been obtained. A test-system has been developed based on polymerase chain reaction for diagnosis and identification of BNYVV with complementary primers to the conserved sequences of gene that encoding the virus coat protein. Also the optimum conditions for PCR have been established. PCR test-systems for segments identification of BNYVV have been developed and plasmid standards for the quantitative analysis of genome expression elements have been created. The genetic features of Ukrainian isolate of BNYVV have been established. The full-size nucleotide sequence of coat protein and partial nucleotide sequence of RNA-1 have been established and deposited into a genetic database.