Topol I. Functional state of gut-associated lymphoid tissue rats in conditions of social stress and modulation of the intestinal microflora

The thesis is devoted to the mechanisms of changes in the functional state of immune structures associated with the mucous membranes of the gastrointestinal tract in conditions of chronic social stress and modulation of the intestinal microflora. We used the combination of immunohistochemical and immunofluorescence techniques, methods of molecular genetic analysis (RТ-PCR-RТ), computer image analysis, statistical methods. We were the first to show that the development of the CSS led to changes in the distribution of TLR2+/TLR4+ - and Nf-kB+lymphocytes, imbalance Th1/Th2 and Treg/Th17-cells, changes in the total number of LMP2+- and ХВР1+- lymphocytes, reducing mRNA expression of Nr3c1 and Adr beta2-receptors and an increase in transcriptional activity of proinflammatory cytokines and Nlrp3-inflammasome genes. Thus, we have established immune mechanisms of proinflammatory signaling activation in GALT under conditions of chronic social stress: (1) activation of the innate immune system; (2) and adaptive components of immune system; (3) violation of T-regulatory level; (4) immuneproteasome defect; (5) changes in the sensitivity of target cells to glucocorticoids and catecholamines; (6) endoplasmic reticulum stress.