Kopich V. Lipoxygenase functioning under the influence of plant hormones and lipoxygenase metabolites

Enzymatic oxidation of polyunsaturated fatty acids by lipoxygenase. Establishment of laws of manifestation of the functional activity of 9- and 13- lipoxygenases using natural compounds and factors - plant hormones, primary lipoxygenase products, abiotic stressors. RP-HPLC, chromatographic methods of purification of proteins and lipids, UV spectrophotometry, enzymatic synthesis, kinetic and mathematical methods. The significant changes in the activity of plant 9- and 13-lipoxygenase at low temperature, high salt concentration and under exogenous 24-epibrassinolide and abscisic acid influence were founded. In model systems of mechanical wounding on potato tubers under conditions of exogenous linoleic acid 13-hydroperoxide or arachidonic acid 15-hydroperoxide application was found a significant decrease in 9-lipoxygenase activity. It was determined that introduction to the structure of 24-epibrassinolide additional active ingredient in the form of balance heteroauxin decreased the IC50 on the order in inhibition of potato 9-lipoxygenase. It was determined the activatory effect of lysophosphatidylinositol and lysophosphatidic acid on oxidation of linoleic acid by potato 9-lipoxygenase. Field of use - bioorganic chemistry, biochemistry, enzymology and biotechnology.