Andriiash G. Preparation of Clostridium strains-producer with elevated level of butanol.

The aim of the thesis - to identify and obtain the method of selection of butanol-producing strains of the genus Clostridium and develop the theoretical foundations of biotechnological process of producing butanol. The object of study - the microbiological synthesis of butanol. Methods study - microbiological (antibiotic sensitivity, the morphological properties of strains), chromatography (to determine the concentration of solvents by gas chromatography), spectrophotometric (determination of biomass concentration of bacteria determination butyric acid concentration) molecular genetic (DNA isolation, PCR analysis), chemical mutagenesis and the statistical processing of the results In thesis obtained new strains-producers of butanol genus Clostridium, studied their biological characteristics, proposed methods of increasing accumulation butanol, show the use of non-food raw materials as substrate and developed the theoretical basis biotechnological process of producing butanol. A microbiological analysis of soil and sludge from Kiev's reservoirs was conducted and clostridial forms of bacteria allocated. The nucleotide sequence of 16S RNA gene strain IFBG C6H was determined and respective phylogenetic trees strains constructed. A fermentation using new strains-producers and lihnocellulose material as the substrate was conducted. An intensification of the synthesis of butanol using chemical mutagenesis was conducted. According to the research were obtained mutant strain IFBG C6H 5M which produced a up 45% butanol to lignocellulosic feedstock (biomass switchgrass Panicum virgatum L).