Antonovych H. Antiviral properties in vitro and in vivo of some of 6H-indolo[2,3-b]quinoxaline derivatives.

Pre-clinical investigation of three newly-synthesized derivatives of 6H-indolo[2,3-b]quinoxaline was conducted. Low toxicity of the compounds in vitro and in vivo, prophylactic antiviral activity against RNA- and DNA-containing viruses in vitro, IFN-inducing potential, both in cell cultures and on rodent models were demonstrated. Compounds' effect upon the immune system of laboratory animals was studied. Tested derivatives influenced production of IFN and other cytokines. Three investigated indoloquinoxalines potentiated phagocytic and metabolic activity of circulating neutrophils, monocytes and peritoneal macrophages, increased the percentage of NK-cells among lymphocytes, which contributed to manifestation of their antiviral effects. Compared to official antiviral drug Amixin, tested derivatives induced prolonged IFN-production with minimal impact on pro-inflammatory markers: number of monocytes and neutrophils in blood, activity of the complement system, the level of monocyte-chemotactic protein-1 and activity of circulating phagocytes. On the model of system infection, caused by Vesicular Stomatitis Indiana virus, and in case of herpetic meningoencephalitis, investigated compounds fully protected animals from sublethal viral doses.