Naumenko O. Pathogenetic mechanisms of rotavirus infection in children, new approaches to treatment

Dissertation is devoted to the improvement of pathogenetic treatment of rotavirus infection (RVI) in children based on the study of metabolic, dysbiotic processes in the intestinal lumen and genetic factors. On the basis of comprehensive studies have identified clinical features of the course on a scale altimetry Vezikari, depending on the age of the sick children and the degree of severity of the infection. Studied the content of active microbial metabolites - volatile fatty acids (butyric, valeric and propionic acid) in children with RVI, proved their role in the course of the disease, evaluated by biochemical markers of worsening lactase insufficiency in children. Pathogenesis RVI supplemented the study activity of lactase by molecular genetic studies and metabolic processes in the intestinal lumen. Studied allelic polymorphism of the lactase gene C> T in position 13910 and analyzed RVI clinic depending on various combinations of carriers of allelic variants of polymorphism. It was found that patients with RVI genotype C / C-13910 was observed in 1.39 times more likely than healthy children. The clinical course of RVI children with genotype C / C-13910, characterized by pronounced intoxication syndrome among children with genotype C / T-13910 marked the longest diarrheal syndrome. Current RVI in patients with genotype T / T-13910 characterized by minimal clinical symptoms corresponding to the mild course of the disease. In children with genotype C / C-13910 risk of severe infection is 1.1 times higher than in children heterozygous C / T 13910, but in children with genotype T / T 13910 chance of developing lung flow RVI 1.4 times higher compared to the native children genotype C / C-13910. Clinical and laboratory proved the efficacy of the combined regimen Saccharomyces boulardii in combination with Lactose-Free Formula in the complex treatment of patients with RVI.